PMS-2D Raw Data Format

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High-precision outdoor Wi-Fi

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Author contributions: M. Water channel aquaporin 4 AQP4 plays a key role in the regulation of water homeostasis in the brain. It is predominantly expressed in astrocytes at the blood—brain and blood—liquor interfaces. Although several AQP4 isoforms have been identified in the mammalian brain, two, AQP4a M1 and AQP4c M23 , have been confirmed to cluster into plasma membrane supramolecular structures, termed orthogonal arrays of particles OAPs and to enhance water transport through the plasma membrane.

However, the role of the newly described water-conductive mammalian isoform AQP4e is unknown. Here, the dynamics of AQP4e aggregation into OAPs and its role in the regulation of astrocyte water homeostasis have been studied. Using super-resolution structured illumination, atomic force, and confocal microscopies, the results revealed that, in female rat astrocytes, AQP4e isoform colocalizes with OAPs, affecting its structural dynamics.

Moreover, the kinetics of the cell swelling and of the regulatory volume decrease was faster in astrocytes overexpressing AQP4e compared with untransfected controls. Furthermore, the increase in maximal cell volume elicited by hypoosmotic stimulation was significantly smaller in AQP4e-overexpressing astrocytes. For the first time, this study demonstrates an active role of AQP4e in the regulation of OAP structural dynamics and in water homeostasis.

We here studied the dynamics, aggregation, and role in the regulation of astrocyte water homeostasis of the newly described water-conductive mammalian isoform AQP4e. Our main findings are as follows: brain edema mimicking hypoosmotic conditions stimulates the formation of new OAPs with larger diameters, due to the incorporation of additional cytoplasmic AQP4 channels and the redistribution of AQP4 channels of the existing OAPs; and AQP4e affects the dynamics of cell swelling and regulatory volume decrease in astrocytes exposed to hypoosmotic conditions.

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The data records will follow and are the same as they have been all along, with the exception that tas is no longer encoded. Additional meta-data may present. The PMS-2D raw data comes in 4 kilo-byte records of 32 bit slices from the probe slices. The interface card then wraps a record header around that with time stamp and other pertinent information.

The Fast2D raw data comes in 4 kilo-byte records of 64 bit slices from the probe slices. The data is stored in big-endian format, so all data will need to be swapped when used on Intel architecture Linux or MS Windows. For ADS2 data, typical values when in heavy cloud should be on the order of 37 milliseconds for 1 probe attached to the VME interface card and milliseconds if 2 probes are attached.

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About The Journal. All submissions can be done at our Online Manuscript Submission System. Authors are requested to submit their articles directly to Online Manuscript Submission. Cell, Cell biology, Cell human genetics, Cell replacement therapy, Cell signalling, Cell systems biology, Cell therapeutics, Cell therapy bioprocessing, Cell therapy products, etc.

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Strapp, J. Albers, A. Reuter, A. Korolev, U.

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